β4  (Cell Signaling Technology Inc)

Journal: eLife

Article Title: Proteomic landscape of tunneling nanotubes reveals CD9 and CD81 tetraspanins as key regulators

doi: 10.7554/eLife.99172

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Article Snippet: Primary antibodies used for Western blot were: mouse anti-CD9 IgG1 (TS9, 1:1000), mouse anti-CD81 IgG2a (TS81, 1:1000), mouse anti-CD63 (1:1000), mouse anti ADAM10 (11G2, 1:1000) ( ; ), rabbit anti-α-GAPDH (Sigma ref: G9545, 1:1000), mouse anti-actin (MP Biomedicals, clone C4, 1:1000), mouse anti-α-tubulin (Sigma ref: T9026, 1:2000), mouse anti-GM130 (BD transduction Laboratories, 610823, 1:1000), rabbit ITGB1, ITGB4, ITGA4 (from integrin Ab Sampler kit, Cell signaling ref: 4749, 1:1000), rabbit anti-EGFR (Cell Signaling ref: 4267, 1:1000), rabbit anti-CX43 (Sigma ref: C6219, 1:3000), mouse anti-ANXA2 (Proteintech ref: 66035, 1:2000), mouse anti-Alix (Biorad MCA2493, 1:1000), rabbit anti-Calnexin (Enzo SPA-860, 1:1000).

Techniques: Transfection, Construct, Expressing, Plasmid Preparation, Marker, Sequencing, Purification, Transduction, Control, Software, Staining

Journal: Hepatology Communications

Article Title: Protective role of 17β-estradiol in alcohol-associated liver fibrosis is mediated by suppression of integrin signaling

doi: 10.1097/HC9.0000000000000428

Figure Lengend Snippet: Estrogen suppresses integrin expression. (A) Relative gene expression in mouse peritoneal macrophages treated in vitro with 10 nM, 100 nM, or 1 µM 17β-estradiol solution. N=3 independent experiments. *, p <0.05, **, p <0.01 by paired t test. (B and C) Female wild-type and Prmt6 KO mice were subjected to gonadectomy (OVX) or sham surgery and placed on WD control or WDA alcohol diet for 18 weeks. Whole liver mRNA. N=3–10 mice per group. *, p <0.05, **, p <0.01 by one-way ANOVA with Bonferroni multiple comparisons test in WT and KO mice separately. (D) Representative images of immunohistochemistry staining in WT female mice using integrin α4 (ITGA4) specific antibody. (E) Relative gene expression in blood monocytes from female and male patients with ALD or HCV *, p <0.05 by Student t test. Abbreviations: ALD, alcohol-associated liver disease; Ilk , integrin-linked kinase; KO, knockout; OVX, ovariectomy; WD, western diet; WDA, western diet with alcohol; WT, wild type.

Article Snippet: Integrin α4 , Cell Signaling , #4749.

Techniques: Expressing, Gene Expression, In Vitro, Control, Immunohistochemistry, Staining, Knock-Out, Western Blot

Journal: Hepatology Communications

Article Title: Protective role of 17β-estradiol in alcohol-associated liver fibrosis is mediated by suppression of integrin signaling

doi: 10.1097/HC9.0000000000000428

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Article Snippet: Integrin α4 , Cell Signaling , #4749.

Techniques:

Journal: Hepatology Communications

Article Title: Protective role of 17β-estradiol in alcohol-associated liver fibrosis is mediated by suppression of integrin signaling

doi: 10.1097/hc9.0000000000000428

Figure Lengend Snippet: FIGURE 2 Female sex hormones protect from alcohol and high-fat diet–induced inflammation and fibrosis. Female wild-type (WT) mice were subjected to gonadectomy (OVX) or sham surgery and placed on WD control diet or WD with alcohol in the drinking water (alternating 10% and 20%) for 18 weeks. (A) Representative images of immunohistochemistry staining using COL1A1, αSMA, and TGFβ1 specific antibodies. (B) Percent positive staining area. N = 3 mice per group *, p < 0.05, **, p < 0.01 by one-way ANOVA with Bonferroni multiple comparisons test. Right.

Article Snippet: PRMT6 Santa Cruz #sc-271744 Integrin α4 Cell Signaling #4749 COL1A1 Cell Signaling #91144 αSMA Cell Signaling #19245 TGFβ1 Cell Signaling #3709 Measurement of liver triglyceride content Triglycerides (TGs) were measured as described.

Techniques: Control, Immunohistochemistry, Staining

Journal: Hepatology Communications

Article Title: Protective role of 17β-estradiol in alcohol-associated liver fibrosis is mediated by suppression of integrin signaling

doi: 10.1097/hc9.0000000000000428

Figure Lengend Snippet: FIGURE 6 Female sex hormones protect from alcohol and high-fat diet-induced inflammation and fibrosis in Prmt6 KO mice. (A and B) Prmt6 knockout mice were subjected to gonadectomy (OVX) or sham surgery and placed on WD control diet or WD with alcohol in the drinking water (alternating 10 and 20%) for 18 weeks. (A and B) Whole liver mRNA N=3–5 mice per group. **, p<0.01 by Student t test. (C) Representative images of immunohistochemistry staining of liver tissue using COL1A1 or αSMA specific antibodies. N=3. *, p<0.05 compared to WD control by student t test. (D) Whole liver mRNA for WT and KO male mice injected with estrogen or control, N=5–10 mice per group. *, p<0.05 by student t test. (E) The ratio of protein abundance in gonadectomy/sham groups (OVX/Sham) in WT and KO mice. (F) GO term biological process enrichment analysis in top upregulated and downregulated proteins. (G) Relative protein abundance of cell adhesion molecules in KO mice subjected to sham (Sham) surgery or gonadectomy (OVX). Abbreviations: αSMA, alpha smooth muscle actin; COL1A1, collagen 1A1; GO, gene ontology; KO, knockout; OVX, ovariectomy; WD, western diet; WDA, western diet with alcohol; WT, wild type.

Article Snippet: PRMT6 Santa Cruz #sc-271744 Integrin α4 Cell Signaling #4749 COL1A1 Cell Signaling #91144 αSMA Cell Signaling #19245 TGFβ1 Cell Signaling #3709 Measurement of liver triglyceride content Triglycerides (TGs) were measured as described.

Techniques: Knock-Out, Control, Immunohistochemistry, Staining, Injection, Quantitative Proteomics, Western Blot